Nutrient regulation of biological nitrogen fixation across the tropical western North Pacific

Zuozhu Wen, Thomas J. Browning, Yihua Cai, Rongbo Dai, Ruifeng Zhang, Chuanjun Du, Ruotong Jiang, Wenfang Lin, Xin Liu, Zhimian Cao, Haizheng Hong, Minhan Dai and Dalin Shi*

Science Advances 

DOI:10.1126/sciadv.abl7564

Published: Feb 2022

Nitrogen fixation is critical for the biological productivity of the ocean, but clear mechanistic controls on this process remain elusive. Here, we investigate the abundance, activity, and drivers of nitrogen-fixing diazotrophs across the tropical western North Pacific. We find a basin-scale coherence of diazotroph abundances and N2 fixation rates with the supply ratio of iron:nitrogen to the upper ocean. Across a threshold of increasing supply ratios, the abundance of nifH genes and N2 fixation rates increased, phosphate concentrations decreased, and bioassay experiments demonstrated evidence for N2 fixation switching from iron to phosphate limitation. In the northern South China Sea, supply ratios were hypothesized to fall around this critical threshold and bioassay experiments suggested colimitation by both iron and phosphate. Our results provide evidence for iron:nitrogen supply ratios being the most important factor in regulating the distribution of N2 fixation across the tropical ocean.

https://www.science.org/doi/10.1126/sciadv.abl7564